Second, mutations at the 4(+)/()4 site of LS isoform 42-nAChR preferentially reduced LS phase function. counterparts or vice versa (2HQT and 4VFL, respectively). Mutant-nAChR constructs (and unmodified controls) were expressed inXenopusoocytes. Acetylcholine concentration-response curves and maximum function were assessed using two-electrode voltage clamp electrophysiology. Surface expression was measured with125I-mAb 295 binding and was used to […]