Excised bands were immersed in 3 ml Optiphase HiSafe 2 liquid scintillant (Wallace-Perkin Elmer) and amount of32P incorporation determined by quantification inside a LS6500 scintillation analyzer (Beckman Coulter). == Circulation cytometry == To determine cell cycle distributions, cell populations for analysis were harvested as appropriate, pelleted by centrifugation and washed in 1 PBS before being […]