Inactive (C1553S) and substrate-trapping (D1521A) mutations were engineered into pcDF1-PTPRD and pGEX-PTPRD constructs using site-directed mutagenesis (Quickchange II XL package from Stratagene) as directed by the product manufacturer. We demonstrated how the SRC proteins tyrosine kinase can be a primary substrate of PTP and, upon suppression of MIM, we noticed adjustments in the phosphorylation position […]