Day: May 19, 2023

´╗┐Immunoprecipitation tests demonstrated that GFP-Parkin was indeed ubiquitylated (Fig. circumstances, HA-Parkin was diffusely localized through the entire cytosol and didn’t overlap with mitochondria, whereas Parkin was quickly recruited towards the mitochondria when HeLa cells had been treated using the mitochondrial uncoupler CCCP (carbonyl cyanide m-chlorophenylhydrazone; Fig. 1 A), as reported by Narendra et al. (2008). […]