The NE-activated ChAT+ T cells travel to macrophages in the spleen and release ACh. pharmacological target, additional studies are necessary since its activity seems to differ from that Mmp11 observed in neurons. strong class=”kwd-title” Keywords: inflammation, macrophage, alpha7 acetylcholine receptor, cholinergic anti-inflammatory response, HIV, inflammatory response, cholinergic receptors 1. Introduction In the 19th century, insight into the role of macrophages in human immunology started to emerge [1]. Initially, they were identified as phagocytic cells. Later, technological advances coupled with the advent of molecular biology, proteomics, and genomic approaches allowed a better understanding of the complexities of these unique warriors that collaborate with the immune system to protect the host. Beyond being entirely phagocytic cells that remove dead cells and cellular or exogenous debris, macrophages secrete pro- and anti-inflammatory mediators that enhance the immune response and ultimately participate in the destruction and removal of exogenous invading Amlodipine aspartic acid impurity agents from the hosts system. However, when this does not happen, the host becomes ill. Inflammation is a formidable response that protects the body from exogenous entities such as pathogens. Inflammation involves the attraction of leukocytes and extravasation of plasmatic proteins in the zones of infection, as well as the activation of these leukocytes and proteins to eliminate the infectious agent. Short-term and controlled inflammation processes are a part of an optimal immune response that allows the body to fight and eliminate potentially pernicious agents effectively. However, uncontrolled inflammation and chronic inflammation are life-threatening and are characteristic of chronic infectious diseases such as human immunodeficiency virus (HIV) infection. Indeed, people infected with HIV suffer from chronic inflammatory processes that promote the appearance of non-acquired immunodeficiency syndrome (AIDS)-related complications [2,3]. Macrophages play a crucial role in HIV-induced inflammation and infection. Upon HIV infection, macrophages release cytokine cascades that promote viral replication, which in turn promotes accumulation Amlodipine aspartic acid impurity of virotoxins (trans-activator of transcription (Tat), negative regulatory factor (Nef), viral protein R kbd ( /kbd Vpr kbd ) /kbd , and envelope glycoprotein GP120 (gp120)) in the blood that further potentiate its viral latency and persistence. Over the past years, understanding of the biology of macrophages has changed dramatically, mainly because of the discovery of their participation in an innate neuroimmune response to control inflammation in mammals. In the early 2000s, Kevin Traceys group [4] made a seminal discovery that changed the way the scientific community looks at the macrophage. They demonstrated that the macrophage is a key player in the cholinergic anti-inflammatory pathway (CAP). An immediate outcome of this important finding was that these cells began to be recognized as a crucial player that mediates anti-inflammatory processes as part of the neuroimmune connection called CAP. For this pathway, the central neural tract is the vagus nerve that, upon stimulation, releases acetylcholine (ACh) to further interact with a cholinergic receptor expressed by macrophagesthe alpha7 nicotinic acetylcholine receptor (7-nAChR) [4,5]. On the basis of this critical new role identified in macrophages, it was possible to interrogate a number of inflammatory processes and diseases, thus facilitating the development of new therapeutic strategies including development of new medical devices and novel therapeutic strategies to Amlodipine aspartic acid impurity activate the CAP [6,7,8] in mammals. Reconciling the information available about studies aimed at understanding the status of CAP in HIV is of utmost importance because the chronic inflammation suffered by these patients continues to be an unresolved problem that urgently needs a solution to avoid the appearance of non-AIDS-related complications (i.e., osteoporosis, cardiovascular disease, neurocognitive deterioration, renal dysfunction, thromboembolic disease, insulin resistance and type II diabetes, cancer, multiple end-organ disease, and frailty [3,9,10,11,12]) in these individuals. The purpose of this review is to present the latest knowledge about the involvement of macrophages in HIV-induced inflammation, together with emerging work examining the cholinergic anti-inflammatory response (CAR) of macrophages in the context of HIV infection. Also, possible therapeutic pharmacological approaches are presented to target the CAR in HIV-related settings. We conclude that more work needs to be done focused on identifying and characterizing the 7-nAChR expressed by macrophages to gain knowledge about its pharmacological properties. It is well known that this receptor interacts with several proteins from the intracellular and transmembrane side, which must undoubtedly influence its activity. The information generated from these.
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