Aliquots (50 l) of the next reagents were put into the wells, 09 g/ml alkaline phosphatase (AP)-conjugated anti-mouse IgG1 (Zymed), 18 g/ml AP-conjugated anti-mouse IgG2a (Zymed) or 1 g/ml horseradish peroxydase (HRP)-conjugated anti-mouse IgA (Zymed). 20 min. Cells present in the user interface of 45% and 675% Percoll had been collected to make use of […]