Cells were fixed in 2% paraformaldehyde (Sigma), permeabilized using Permeabilization buffer (Perm, eBioscience) and incubated for 40 moments with anti-IFN–FITC (XMG1.2), IL-10-PE (JES5-16E3), T-bet-efluor 660 (eBio4B10, eBioscience), or Bcl6-Alexa Fluor 488 (K112-91). For IL-21 staining, cells were incubated for 40 minutes with recombinant mouse IL-21R-Fc chimera (1 g, R&D systems, Minneapolis, MN) in Perm and […]