Therefore, a variety of engineered materials are being explored for human-derived and animal-derived organoid cultures (TABLE 1), including organotypic cultures157C160. Table 1 | Materials systems for organoids can be added to model pathogen attack on the intestine. the reproducible generation and control of organoid cultures. We survey natural, synthetic and protein-engineered hydrogels for their applicability to different organoid systems and discuss biochemical and mechanical material properties relevant for organoid formation. Finally, dynamic and cell-responsive material systems are investigated for their future use in organoid research. Organoids are 3D cell culture systems that are formed through cell differentiation and self-organization of pluripotent stem cells or tissue-derived progenitor cells, which can contain supporting stromal elements. The foundation of tissue culture was laid in 1907, when Harrison et al. cultured dissected frog neural tubes1. Cell culture studies were continued throughout the 20th century to describe the embryonic development of organs by observing tissue reorganization after dissociation2,3 (FIG. 1 ), which led to the identification of cell sorting and cell-fate specification during organogenesis and the powerful innate ability of cells to spontaneously organize into complex structures in vitro. Organoids are a class of microphysiological systems that provide platforms to model the features of organs and tissues in an in vitro setting4. The terminology in the field remains to be universally defined5 and terms such as organoid, organotypic culture, spheroid, enteroid and assembloid are used by different communities for different 3D cell culture systems. For example, for gastrointestinal tissues, the term organoid has been suggested for cultures that contain both epithelial and mesenchymal or stromal components, whereas the term enteroid has been used for 3D cultures that contain only epithelial cells6. By contrast, spheroid has been used to describe either aggregates of cells or region-specific brain organoids7. In this Review, the term organoid is used to describe all of these complex, multicellular systems. Open in a separate window Fig. 1 | Timeline of milestones for biomaterials, organoids and stem cells.PEG, polyethylene glycol. Microphysiological systems usually contain two or more interacting cell types, which are in contact with each other and embedded in a matrix (either cell-secreted or externally introduced) or in a device with the aim to partially mimic cellular interactions and/or functions of a tissue or organ in vitro. These systems represent an important intermediary between conventional 2D cell culture systems and animal models, allowing the precise and reproducible investigation of the effects of experimental conditions on cell and tissue behaviour. Organoid cultures have great potential to transform drug development and disease research, as drug tests and disease studies have traditionally mostly relied on 2D in vitro cell culture assays or animal models. 2D cell culture models are simple and have a high throughput but they fail to capture the physiological complexity of entire tissues and organisms8,9. In particular, the modelling of brain development remains challenging, as this process requires months to MK-1064 years in humans and other primates, which is difficult to recreate in 2D in vitro cultures10. Animal models are important for basic and applied research but are time consuming, expensive and often limited by species-specific anatomy and physiology, which can make them less relevant for the investigation of human biology FSCN1 and pathology11,12. Advances in cell biology, biomaterials design and imaging techniques have enabled the investigation of increasingly complex biological questions; however, a gap remains between single-cell-type culture systems and actual tissues. Therefore, more sophisticated and physiologically relevant in vitro tissue models are required to study human biology and medicine13C15. Organoids have the advantage of being based on human cells cultured in a physiologically meaningful context, that is, multiple interacting cell types with spatial organization. In contrast to other microphysiological platforms, such as organ-on-a-chip culture systems, in which cellular organization is externally imposed and nutrient supply and physiological levels of shear forces are achieved by using microfluidic chambers16, organoids are typically cultured in static 3D conditions, in which cells self-assemble into multicellular entities with an architecture similar to real tissues. By contrast, in organ-on-a-chip systems, differentiated cells are usually MK-1064 placed at specific regions within a device, which does not allow higher-level cell sorting or ordering16,17. However, organ-on-a-chip platforms and organoid cultures both strive to accurately model physiological behaviours that require multicellular interactions, and they can be combined by incorporating cellular spheroids and organoids MK-1064 into organ-on-a-chip systems18C21. Organoid cultures typically arise from stem cells that undergo proliferation, differentiation and self-organization22,23. Organoid generation can, in principle, be scaled up, making.
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